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When the flow rate was changed, it was found that a flow rate of 1 mL min -1 gave the best results for getting resolved peaks with the least amount of solvent use and run time

Understanding how they interact, when combining them makes sense, and when it doesnt

It needs to be long enough to reach deep into the muscle tissue

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Regardless, it is unlikely to be the only mechanism at work and additional mechanisms operating at the synaptic, cellular, and network levels might be involved in homeostatic regulation of activation of inhibitory neurons